Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3731540..3732177 Replicon chromosome
Accession NZ_CP097591
Organism Bacillus velezensis strain UA0188

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8962_RS18470 Protein ID WP_003156187.1
Coordinates 3731827..3732177 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8962_RS18465 Protein ID WP_003156188.1
Coordinates 3731540..3731821 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8962_RS18445 3727905..3728504 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
M8962_RS18450 3728597..3728962 + 366 WP_012116869.1 holo-ACP synthase -
M8962_RS18455 3729127..3730134 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
M8962_RS18460 3730251..3731420 + 1170 WP_012116870.1 alanine racemase -
M8962_RS18465 3731540..3731821 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8962_RS18470 3731827..3732177 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8962_RS18475 3732295..3733116 + 822 WP_003156182.1 STAS domain-containing protein -
M8962_RS18480 3733121..3733486 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8962_RS18485 3733489..3733890 + 402 WP_003156178.1 anti-sigma regulatory factor -
M8962_RS18490 3733902..3734909 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
M8962_RS18495 3734973..3735302 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8962_RS18500 3735299..3735781 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M8962_RS18505 3735747..3736535 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M8962_RS18510 3736535..3737137 + 603 WP_012116871.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245646 WP_003156187.1 NZ_CP097591:3731827-3732177 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245646 WP_003156188.1 NZ_CP097591:3731540-3731821 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References