Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3654901..3655538 Replicon chromosome
Accession NZ_CP097590
Organism Bacillus velezensis strain UA0195

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8970_RS17875 Protein ID WP_003156187.1
Coordinates 3655188..3655538 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8970_RS17870 Protein ID WP_003156188.1
Coordinates 3654901..3655182 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8970_RS17850 3651266..3651865 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
M8970_RS17855 3651958..3652323 + 366 WP_007609580.1 holo-ACP synthase -
M8970_RS17860 3652488..3653495 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
M8970_RS17865 3653612..3654781 + 1170 WP_032873001.1 alanine racemase -
M8970_RS17870 3654901..3655182 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8970_RS17875 3655188..3655538 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8970_RS17880 3655656..3656477 + 822 WP_003156182.1 STAS domain-containing protein -
M8970_RS17885 3656482..3656847 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8970_RS17890 3656850..3657251 + 402 WP_003156178.1 anti-sigma regulatory factor -
M8970_RS17895 3657263..3658270 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
M8970_RS17900 3658334..3658663 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8970_RS17905 3658660..3659142 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M8970_RS17910 3659108..3659896 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
M8970_RS17915 3659896..3660498 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245644 WP_003156187.1 NZ_CP097590:3655188-3655538 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245644 WP_003156188.1 NZ_CP097590:3654901-3655182 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References