Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2115296..2115933 Replicon chromosome
Accession NZ_CP097589
Organism Bacillus velezensis strain UA0311

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8966_RS09965 Protein ID WP_003156187.1
Coordinates 2115583..2115933 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8966_RS09960 Protein ID WP_003156188.1
Coordinates 2115296..2115577 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8966_RS09940 2111661..2112260 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
M8966_RS09945 2112353..2112718 + 366 WP_012116869.1 holo-ACP synthase -
M8966_RS09950 2112883..2113890 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
M8966_RS09955 2114007..2115176 + 1170 WP_012116870.1 alanine racemase -
M8966_RS09960 2115296..2115577 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8966_RS09965 2115583..2115933 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8966_RS09970 2116051..2116872 + 822 WP_003156182.1 STAS domain-containing protein -
M8966_RS09975 2116877..2117242 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8966_RS09980 2117245..2117646 + 402 WP_003156178.1 anti-sigma regulatory factor -
M8966_RS09985 2117658..2118665 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
M8966_RS09990 2118729..2119058 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8966_RS09995 2119055..2119537 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M8966_RS10000 2119503..2120291 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M8966_RS10005 2120291..2120893 + 603 WP_012116871.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245642 WP_003156187.1 NZ_CP097589:2115583-2115933 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245642 WP_003156188.1 NZ_CP097589:2115296-2115577 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References