Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1749915..1750552 Replicon chromosome
Accession NZ_CP097588
Organism Bacillus velezensis strain UA0323

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8961_RS08485 Protein ID WP_003156187.1
Coordinates 1749915..1750265 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8961_RS08490 Protein ID WP_003156188.1
Coordinates 1750271..1750552 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8961_RS08445 1744955..1745557 - 603 WP_012116871.1 PP2C family serine/threonine-protein phosphatase -
M8961_RS08450 1745557..1746345 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M8961_RS08455 1746311..1746793 - 483 WP_007609591.1 anti-sigma B factor RsbW -
M8961_RS08460 1746790..1747119 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8961_RS08465 1747183..1748190 - 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
M8961_RS08470 1748202..1748603 - 402 WP_003156178.1 anti-sigma regulatory factor -
M8961_RS08475 1748606..1748971 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8961_RS08480 1748976..1749797 - 822 WP_003156182.1 STAS domain-containing protein -
M8961_RS08485 1749915..1750265 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8961_RS08490 1750271..1750552 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8961_RS08495 1750672..1751841 - 1170 WP_012116870.1 alanine racemase -
M8961_RS08500 1751958..1752965 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
M8961_RS08505 1753130..1753495 - 366 WP_012116869.1 holo-ACP synthase -
M8961_RS08510 1753588..1754187 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245640 WP_003156187.1 NZ_CP097588:c1750265-1749915 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245640 WP_003156188.1 NZ_CP097588:c1750552-1750271 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References