Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 124972..125609 Replicon chromosome
Accession NZ_CP097587
Organism Bacillus velezensis strain UA0276

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8957_RS00690 Protein ID WP_003156187.1
Coordinates 124972..125322 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8957_RS00695 Protein ID WP_003156188.1
Coordinates 125328..125609 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8957_RS00650 120012..120614 - 603 WP_012116871.1 PP2C family serine/threonine-protein phosphatase -
M8957_RS00655 120614..121402 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M8957_RS00660 121368..121850 - 483 WP_007609591.1 anti-sigma B factor RsbW -
M8957_RS00665 121847..122176 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8957_RS00670 122240..123247 - 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
M8957_RS00675 123259..123660 - 402 WP_003156178.1 anti-sigma regulatory factor -
M8957_RS00680 123663..124028 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8957_RS00685 124033..124854 - 822 WP_003156182.1 STAS domain-containing protein -
M8957_RS00690 124972..125322 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8957_RS00695 125328..125609 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8957_RS00700 125729..126898 - 1170 WP_012116870.1 alanine racemase -
M8957_RS00705 127015..128022 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
M8957_RS00710 128187..128552 - 366 WP_012116869.1 holo-ACP synthase -
M8957_RS00715 128645..129244 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245637 WP_003156187.1 NZ_CP097587:c125322-124972 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245637 WP_003156188.1 NZ_CP097587:c125609-125328 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References