245635

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1199855..1200492 Replicon chromosome
Accession NZ_CP097585
Organism Bacillus velezensis strain UA1365

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8964_RS06035 Protein ID WP_003156187.1
Coordinates 1200142..1200492 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8964_RS06030 Protein ID WP_003156188.1
Coordinates 1199855..1200136 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8964_RS06010 1196220..1196819 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
M8964_RS06015 1196912..1197277 + 366 WP_012116869.1 holo-ACP synthase -
M8964_RS06020 1197442..1198449 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
M8964_RS06025 1198566..1199735 + 1170 WP_012116870.1 alanine racemase -
M8964_RS06030 1199855..1200136 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8964_RS06035 1200142..1200492 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8964_RS06040 1200610..1201431 + 822 WP_003156182.1 STAS domain-containing protein -
M8964_RS06045 1201436..1201801 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8964_RS06050 1201804..1202205 + 402 WP_003156178.1 anti-sigma regulatory factor -
M8964_RS06055 1202217..1203224 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
M8964_RS06060 1203288..1203617 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8964_RS06065 1203614..1204096 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M8964_RS06070 1204062..1204850 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M8964_RS06075 1204850..1205452 + 603 WP_012116871.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245635 WP_003156187.1 NZ_CP097585:1200142-1200492 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245635 WP_003156188.1 NZ_CP097585:1199855-1200136 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References