Detailed information of TA system TA245564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5376712..5378432
• Replicon: chromosome
• Accession: NZ_CP097554
• Organism: Klebsiella michiganensis strain YZUMF202001

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: M8333_RS25335
• Protein ID: WP_151585550.1
• Coordinates: 5377776..5378432 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: M8333_RS25330
• Protein ID: WP_151585547.1
• Coordinates: 5376712..5377779 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M8333_RS25310 (M8333_25305)	5372253..5372462	+	210	WP_267313341.1	hypothetical protein	-
M8333_RS25315 (M8333_25310)	5372526..5372687	+	162	Protein_4975	VOC family protein	-
M8333_RS25320 (M8333_25315)	5372880..5373596	-	717	WP_151585540.1	SprT family zinc-dependent metalloprotease	-
M8333_RS25325 (M8333_25320)	5373596..5376691	-	3096	WP_151585544.1	HsdR family type I site-specific deoxyribonuclease	-
M8333_RS25330 (M8333_25325)	5376712..5377779	-	1068	WP_151585547.1	macro domain-containing protein	Antitoxin
M8333_RS25335 (M8333_25330)	5377776..5378432	-	657	WP_151585550.1	DUF4433 domain-containing protein	Toxin
M8333_RS25340 (M8333_25335)	5378445..5379758	-	1314	WP_151585553.1	restriction endonuclease subunit S	-
M8333_RS25345 (M8333_25340)	5379758..5382196	-	2439	WP_151585556.1	type I restriction-modification system subunit M	-
M8333_RS25350 (M8333_25345)	5382236..5382475	-	240	WP_012543063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5347107..5397174	50067

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25330.73 Da
• Isoelectric Point: 6.4009

>T245564 WP_151585550.1 NZ_CP097554:c5378432-5377776 [Klebsiella michiganensis]
MAYDYSANLNPEKALIWRIVHRDNIPWILDNGLHCGNSQVQAENWVNIGNPELIDKRAGHPVPAGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIMQRPNEEIVILVSSLRHVAAQNVPFVFTDSHAYYNWTNYYTRLDDLNQIDWPMLQARDFRRDPDD
PAKFERYQAEALIWKHCPITLLSGMVCYDNDVRLQLEQWLLQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40968.20 Da
• Isoelectric Point: 6.0616

>AT245564 WP_151585547.1 NZ_CP097554:c5377779-5376712 [Klebsiella michiganensis]
MMKYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPDNMKSYALACKQKQVMTGKMFITETSELMGPRWIVNFPTK
QHWRADSRMEWIEEGLQDLRRFLIEEKVQSIAIPPLGAGNGGLNWPDVRARIESALGDLQDTEILIYEPTEKYQNVAKNT
GVKKLTPARAMIAELVRRYWVQGMECSLLEIQKLAWLLQRAIELHQQEDVLKLRFEAHNYGPFSPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDREKEHVNLWLNNEAKEWLPALEQVSQLIDGFESPFGLELLATVDWLLTRGECQPTLDSVKEG
LHQWPAGERWASRKMKLFDDHHLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp