Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5294676..5295252 Replicon chromosome
Accession NZ_CP097554
Organism Klebsiella michiganensis strain YZUMF202001

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag M8333_RS24925 Protein ID WP_042933975.1
Coordinates 5294965..5295252 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1D8JNF7
Locus tag M8333_RS24920 Protein ID WP_025108476.1
Coordinates 5294676..5294978 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8333_RS24905 (M8333_24900) 5291515..5291850 + 336 WP_047724615.1 endoribonuclease SymE -
M8333_RS24910 (M8333_24905) 5292304..5293215 + 912 WP_128696653.1 acetamidase/formamidase family protein -
M8333_RS24915 (M8333_24910) 5293212..5294555 + 1344 WP_025108477.1 APC family permease -
M8333_RS24920 (M8333_24915) 5294676..5294978 - 303 WP_025108476.1 BrnA antitoxin family protein Antitoxin
M8333_RS24925 (M8333_24920) 5294965..5295252 - 288 WP_042933975.1 BrnT family toxin Toxin
M8333_RS24930 (M8333_24925) 5295504..5295947 - 444 WP_025108475.1 FosA family fosfomycin resistance glutathione transferase -
M8333_RS24935 (M8333_24930) 5295941..5296849 - 909 WP_025108474.1 LysR family transcriptional regulator -
M8333_RS24940 (M8333_24935) 5296937..5297719 + 783 WP_025108473.1 NAD(P)H-dependent oxidoreductase -
M8333_RS24945 (M8333_24940) 5297867..5298451 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
M8333_RS24950 (M8333_24945) 5298597..5299397 + 801 WP_014227752.1 winged helix-turn-helix domain-containing protein -
M8333_RS24955 (M8333_24950) 5299394..5299912 + 519 WP_004098240.1 FidL-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T245563 WP_042933975.1 NZ_CP097554:c5295252-5294965 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11563.17 Da        Isoelectric Point: 9.7962

>AT245563 WP_025108476.1 NZ_CP097554:c5294978-5294676 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8JNF7

References