245556

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2094328..2094918 Replicon chromosome
Accession NZ_CP097554
Organism Klebsiella michiganensis strain YZUMF202001

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag M8333_RS09955 Protein ID WP_014229979.1
Coordinates 2094586..2094918 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag M8333_RS09950 Protein ID WP_004852307.1
Coordinates 2094328..2094585 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8333_RS09925 (M8333_09930) 2089585..2090190 - 606 WP_004852315.1 glutathione S-transferase family protein -
M8333_RS09930 (M8333_09935) 2090370..2091296 + 927 WP_049101399.1 LysR substrate-binding domain-containing protein -
M8333_RS09935 (M8333_09940) 2091334..2092332 - 999 WP_025106069.1 aldo/keto reductase -
M8333_RS09940 (M8333_09945) 2092433..2093347 + 915 WP_025106070.1 LysR family transcriptional regulator -
M8333_RS09945 (M8333_09950) 2093466..2093735 - 270 WP_185220221.1 hypothetical protein -
M8333_RS09950 (M8333_09955) 2094328..2094585 + 258 WP_004852307.1 antitoxin Antitoxin
M8333_RS09955 (M8333_09960) 2094586..2094918 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M8333_RS09965 (M8333_09970) 2095241..2096698 + 1458 WP_128334953.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
M8333_RS09975 (M8333_09980) 2097661..2099115 - 1455 WP_014229909.1 AMP nucleosidase -
M8333_RS09980 (M8333_09985) 2099248..2099505 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2084623..2094918 10295


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T245556 WP_014229979.1 NZ_CP097554:2094586-2094918 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT245556 WP_004852307.1 NZ_CP097554:2094328-2094585 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References