Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 91602..92214 Replicon plasmid pDSHI03
Accession NZ_CP097543
Organism Dinoroseobacter shibae strain DSM 112351

Toxin (Protein)


Gene name vapC Uniprot ID A0A840CKU8
Locus tag M8008_RS20640 Protein ID WP_012187367.1
Coordinates 91819..92214 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag M8008_RS20635 Protein ID WP_012187366.1
Coordinates 91602..91829 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8008_RS20620 (M8008_20620) 87675..88910 - 1236 WP_012187363.1 plasmid replication protein RepC -
M8008_RS20625 (M8008_20625) 89088..90104 - 1017 WP_012187364.1 plasmid partitioning protein RepB -
M8008_RS20630 (M8008_20630) 90101..91288 - 1188 WP_012187365.1 plasmid partitioning protein RepA -
M8008_RS20635 (M8008_20635) 91602..91829 + 228 WP_012187366.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M8008_RS20640 (M8008_20640) 91819..92214 + 396 WP_012187367.1 PIN domain-containing protein Toxin
M8008_RS20645 (M8008_20645) 92198..93094 - 897 WP_012187368.1 recombinase family protein -
M8008_RS20650 (M8008_20650) 93153..94271 - 1119 WP_012187369.1 tyrosine-type recombinase/integrase -
M8008_RS20655 (M8008_20655) 94398..95282 + 885 WP_012187056.1 DUF1403 family protein -
M8008_RS20660 (M8008_20660) 95285..95902 + 618 WP_012187055.1 SMC-Scp complex subunit ScpB -
M8008_RS20665 (M8008_20665) 95952..96215 + 264 WP_012187054.1 WGR domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..126304 126304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-113)

Antitoxin

SpoVT-AbrB domain

(5-42)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14296.32 Da        Isoelectric Point: 4.2692

>T245549 WP_012187367.1 NZ_CP097543:91819-92214 [Dinoroseobacter shibae]
MSAEFADTNVVLYLLDDGPKAERAEEILGQGPRISVQVLNEAMVNCRRKAGLSWEDTGAFLEGITSVCPVEGLTVQTHQV
GRALAEKYQLSVYDAMIVSAALIAGCTTLWTEDMHDGLLVEDRLRIVNPFA

Download         Length: 396 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8599.92 Da        Isoelectric Point: 11.1585

>AT245549 WP_012187366.1 NZ_CP097543:91602-91829 [Dinoroseobacter shibae]
MQVAKWGNSLAVRLPADLVRELGLKEGDQVDLVKDDGTILVRRQPRADEVLQGLRRFRRKLPKDARLSRDAAHER

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A840CKU8

Antitoxin

Source ID Structure

References