Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 2780270..2781153 Replicon chromosome
Accession NZ_CP097528
Organism Pseudomonas putida strain KT2440 isolate pMBEC6

Toxin (Protein)


Gene name - Uniprot ID Q88K57
Locus tag M8003_RS12770 Protein ID WP_003250527.1
Coordinates 2780716..2781153 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID I7C3Q7
Locus tag M8003_RS12765 Protein ID WP_003250525.1
Coordinates 2780270..2780719 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8003_RS12740 (M8003_12720) 2775694..2776893 + 1200 WP_049587621.1 sugar transporter -
M8003_RS12745 (M8003_12725) 2776933..2777517 - 585 WP_010953382.1 LysE family transporter -
M8003_RS12750 (M8003_12730) 2777568..2778398 - 831 WP_003250518.1 AraC family transcriptional regulator -
M8003_RS12755 (M8003_12735) 2778447..2779370 - 924 WP_010953383.1 LysR family transcriptional regulator -
M8003_RS12760 (M8003_12740) 2779474..2780127 + 654 WP_010953384.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
M8003_RS12765 (M8003_12745) 2780270..2780719 + 450 WP_003250525.1 DUF2384 domain-containing protein Antitoxin
M8003_RS12770 (M8003_12750) 2780716..2781153 + 438 WP_003250527.1 RES family NAD+ phosphorylase Toxin
M8003_RS12775 (M8003_12755) 2781161..2782315 - 1155 WP_049587669.1 aminotransferase class V-fold PLP-dependent enzyme -
M8003_RS12780 (M8003_12760) 2782509..2783435 - 927 WP_010953387.1 LysR family transcriptional regulator -
M8003_RS12785 (M8003_12765) 2783576..2784805 + 1230 WP_049587623.1 acyl-CoA dehydrogenase -
M8003_RS12790 (M8003_12770) 2784969..2785217 + 249 Protein_2482 PAAR domain-containing protein -
M8003_RS12795 (M8003_12775) 2785220..2785609 + 390 WP_049587628.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15990.29 Da        Isoelectric Point: 5.6007

>T245534 WP_003250527.1 NZ_CP097528:2780716-2781153 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Download         Length: 438 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16956.46 Da        Isoelectric Point: 5.7258

>AT245534 WP_003250525.1 NZ_CP097528:2780270-2780719 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GW6

Antitoxin

Source ID Structure
AlphaFold DB I7C3Q7

References