Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 2780267..2781150 Replicon chromosome
Accession NZ_CP097527
Organism Pseudomonas putida strain KT2440 isolate pnCas9-BEC6-C

Toxin (Protein)


Gene name - Uniprot ID Q88K57
Locus tag M8001_RS12770 Protein ID WP_003250527.1
Coordinates 2780713..2781150 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID I7C3Q7
Locus tag M8001_RS12765 Protein ID WP_003250525.1
Coordinates 2780267..2780716 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8001_RS12740 (M8001_12720) 2775691..2776890 + 1200 WP_049587621.1 sugar transporter -
M8001_RS12745 (M8001_12725) 2776930..2777514 - 585 WP_010953382.1 LysE family transporter -
M8001_RS12750 (M8001_12730) 2777565..2778395 - 831 WP_003250518.1 AraC family transcriptional regulator -
M8001_RS12755 (M8001_12735) 2778444..2779367 - 924 WP_010953383.1 LysR family transcriptional regulator -
M8001_RS12760 (M8001_12740) 2779471..2780124 + 654 WP_010953384.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
M8001_RS12765 (M8001_12745) 2780267..2780716 + 450 WP_003250525.1 DUF2384 domain-containing protein Antitoxin
M8001_RS12770 (M8001_12750) 2780713..2781150 + 438 WP_003250527.1 RES family NAD+ phosphorylase Toxin
M8001_RS12775 (M8001_12755) 2781158..2782312 - 1155 WP_049587669.1 aminotransferase class V-fold PLP-dependent enzyme -
M8001_RS12780 (M8001_12760) 2782506..2783432 - 927 WP_010953387.1 LysR family transcriptional regulator -
M8001_RS12785 (M8001_12765) 2783573..2784802 + 1230 WP_049587623.1 acyl-CoA dehydrogenase -
M8001_RS12790 (M8001_12770) 2784966..2785214 + 249 Protein_2482 PAAR domain-containing protein -
M8001_RS12795 (M8001_12775) 2785217..2785606 + 390 WP_049587628.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-138)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15990.29 Da        Isoelectric Point: 5.6007

>T245525 WP_003250527.1 NZ_CP097527:2780713-2781150 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAQPELTRTLGNRFLDDCSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Download         Length: 438 bp

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16956.46 Da        Isoelectric Point: 5.7258

>AT245525 WP_003250525.1 NZ_CP097527:2780267-2780716 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Download         Length: 450 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GW6

Antitoxin

Source ID Structure
AlphaFold DB I7C3Q7

References