Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-higA/HigB-HigA
Location 332741..333732 Replicon chromosome
Accession NZ_CP097527
Organism Pseudomonas putida strain KT2440 isolate pnCas9-BEC6-C

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag M8001_RS01445 Protein ID WP_020190010.1
Coordinates 332741..333217 (+) Length 159 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q88R60
Locus tag M8001_RS01450 Protein ID WP_004575922.1
Coordinates 333259..333732 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8001_RS01425 (M8001_01420) 327884..329251 - 1368 WP_010951639.1 ATP-binding protein -
M8001_RS01430 (M8001_01425) 329253..329972 - 720 WP_010951640.1 response regulator -
M8001_RS01435 (M8001_01430) 330113..332410 + 2298 WP_010951641.1 TonB-dependent siderophore receptor -
M8001_RS01440 (M8001_01435) 332476..332682 - 207 WP_014860378.1 DUF3079 domain-containing protein -
M8001_RS01445 (M8001_01440) 332741..333217 + 477 WP_020190010.1 type II toxin-antitoxin system HigB family toxin Toxin
M8001_RS01450 (M8001_01445) 333259..333732 + 474 WP_004575922.1 transcriptional regulator Antitoxin
M8001_RS01455 (M8001_01450) 333737..333924 - 188 Protein_282 integrase -
M8001_RS01460 (M8001_01455) 334152..334367 + 216 WP_010951644.1 type II toxin-antitoxin system RelE/ParE family toxin -
M8001_RS01465 (M8001_01460) 334364..334687 - 324 WP_010951645.1 helix-turn-helix transcriptional regulator -
M8001_RS01475 (M8001_01470) 335149..335397 - 249 WP_010951646.1 hypothetical protein -
M8001_RS01480 (M8001_01475) 335918..336193 + 276 WP_049588069.1 DUF3077 domain-containing protein -
M8001_RS01485 (M8001_01480) 336548..337753 - 1206 WP_010951648.1 methyltransferase -
M8001_RS01490 (M8001_01485) 337882..338571 - 690 WP_010951649.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(114-152)

Antitoxin

Antitoxin HigA

(43-154)


Sequences

Toxin        


Download         Length: 159 a.a.        Molecular weight: 18386.38 Da        Isoelectric Point: 10.3522

>T245521 WP_020190010.1 NZ_CP097527:332741-333217 [Pseudomonas putida]
MTGINPLAIARSPYIAHPDIWLTDIWIGDIFHPMLVRRDNTMRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFES
FEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIKKNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Download         Length: 477 bp

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17229.67 Da        Isoelectric Point: 4.4982

>AT245521 WP_004575922.1 NZ_CP097527:333259-333732 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Download         Length: 474 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CBJ4

References