245506

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 73779..74472 Replicon plasmid pBYT5-2
Accession NZ_CP097491
Organism Pseudomonas sp. BYT-5

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag M6G63_RS27425 Protein ID WP_003151133.1
Coordinates 73779..74156 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag M6G63_RS27430 Protein ID WP_001172026.1
Coordinates 74137..74472 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M6G63_RS27410 (M6G63_27410) 69031..69948 + 918 Protein_78 Tn3 family transposase -
M6G63_RS27415 (M6G63_27415) 69972..73001 - 3030 WP_010799689.1 Tn3 family transposase -
M6G63_RS27420 (M6G63_27420) 72985..73587 - 603 WP_010465829.1 recombinase family protein -
M6G63_RS27425 (M6G63_27425) 73779..74156 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M6G63_RS27430 (M6G63_27430) 74137..74472 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
M6G63_RS27435 (M6G63_27435) 74487..74822 + 336 WP_000741275.1 hypothetical protein -
M6G63_RS27440 (M6G63_27440) 74846..75172 + 327 WP_000091614.1 hypothetical protein -
M6G63_RS27445 (M6G63_27445) 75188..75529 + 342 WP_025999701.1 hypothetical protein -
M6G63_RS27450 (M6G63_27450) 75569..76837 + 1269 Protein_86 Tn3 family transposase -
M6G63_RS27455 (M6G63_27455) 76834..77274 - 441 WP_250035703.1 DUF1924 domain-containing protein -
M6G63_RS27460 (M6G63_27460) 77415..79010 + 1596 WP_250035718.1 2Fe-2S iron-sulfur cluster-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..136400 136400
- inside IScluster/Tn - - 69061..81408 12347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T245506 WP_003151133.1 NZ_CP097491:73779-74156 [Pseudomonas sp. BYT-5]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT245506 WP_001172026.1 NZ_CP097491:74137-74472 [Pseudomonas sp. BYT-5]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References