Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 478179..478816 Replicon chromosome
Accession NZ_CP097467
Organism Bacillus velezensis strain HMB26553

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8X21_RS02430 Protein ID WP_003156187.1
Coordinates 478466..478816 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8X21_RS02425 Protein ID WP_003156188.1
Coordinates 478179..478460 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8X21_RS02405 (M8X21_02405) 474544..475143 - 600 WP_029974099.1 rhomboid family intramembrane serine protease -
M8X21_RS02410 (M8X21_02410) 475236..475601 + 366 WP_007609580.1 holo-ACP synthase -
M8X21_RS02415 (M8X21_02415) 475766..476773 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
M8X21_RS02420 (M8X21_02420) 476890..478059 + 1170 WP_021495079.1 alanine racemase -
M8X21_RS02425 (M8X21_02425) 478179..478460 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8X21_RS02430 (M8X21_02430) 478466..478816 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8X21_RS02435 (M8X21_02435) 478934..479755 + 822 WP_003156182.1 STAS domain-containing protein -
M8X21_RS02440 (M8X21_02440) 479760..480125 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
M8X21_RS02445 (M8X21_02445) 480128..480529 + 402 WP_003156178.1 anti-sigma regulatory factor -
M8X21_RS02450 (M8X21_02450) 480541..481548 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
M8X21_RS02455 (M8X21_02455) 481612..481941 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8X21_RS02460 (M8X21_02460) 481938..482420 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M8X21_RS02465 (M8X21_02465) 482386..483174 + 789 WP_029974100.1 RNA polymerase sigma factor SigB -
M8X21_RS02470 (M8X21_02470) 483174..483776 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245496 WP_003156187.1 NZ_CP097467:478466-478816 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245496 WP_003156188.1 NZ_CP097467:478179-478460 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References