Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4251193..4251690 Replicon chromosome
Accession NZ_CP097430
Organism Escherichia coli strain C288

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M8Y68_RS20815 Protein ID WP_001173343.1
Coordinates 4251193..4251366 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M8Y68_RS20820 Protein ID WP_000398619.1
Coordinates 4251394..4251690 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y68_RS20805 (4247863) 4247863..4249320 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
M8Y68_RS20810 (4249557) 4249557..4251074 + 1518 WP_001295074.1 lysine--tRNA ligase -
M8Y68_RS20815 (4251193) 4251193..4251366 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M8Y68_RS20820 (4251394) 4251394..4251690 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M8Y68_RS20825 (4251917) 4251917..4252189 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
M8Y68_RS20830 (4252201) 4252201..4252431 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
M8Y68_RS20835 (4252612) 4252612..4254243 + 1632 WP_001216477.1 sensor histidine kinase -
M8Y68_RS20840 (4254240) 4254240..4254959 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T245484 WP_001173343.1 NZ_CP097430:c4251366-4251193 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT245484 WP_000398619.1 NZ_CP097430:c4251690-4251394 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References