Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4020396..4020972 Replicon chromosome
Accession NZ_CP097430
Organism Escherichia coli strain C288

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M8Y68_RS19640 Protein ID WP_001297643.1
Coordinates 4020685..4020972 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M8Y68_RS19635 Protein ID WP_000063156.1
Coordinates 4020396..4020698 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y68_RS19620 (4016992) 4016992..4019142 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M8Y68_RS19625 (4019192) 4019192..4019395 + 204 WP_000467859.1 YbdD/YjiX family protein -
M8Y68_RS19630 (4019406) 4019406..4020362 + 957 WP_001297640.1 GTPase -
M8Y68_RS19635 (4020396) 4020396..4020698 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M8Y68_RS19640 (4020685) 4020685..4020972 - 288 WP_001297643.1 BrnT family toxin Toxin
M8Y68_RS19645 (4021171) 4021171..4021868 - 698 Protein_3858 IS1 family transposase -
M8Y68_RS19650 (4021925) 4021925..4022020 + 96 Protein_3859 dienelactone hydrolase family protein -
M8Y68_RS19655 (4022166) 4022166..4023398 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
M8Y68_RS19660 (4023439) 4023439..4024719 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T245483 WP_001297643.1 NZ_CP097430:c4020972-4020685 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT245483 WP_000063156.1 NZ_CP097430:c4020698-4020396 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References