Detailed information of TA system TA024544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 315277..315863
• Replicon: chromosome
• Accession: NC_013850
• Organism: Klebsiella variicola At-22

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: KVAR_RS01420
• Protein ID: WP_012967118.1
• Coordinates: 315495..315863 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: KVAR_RS01415
• Protein ID: WP_004174006.1
• Coordinates: 315277..315498 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KVAR_RS01395	311426..312352	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
KVAR_RS01400	312349..313626	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
KVAR_RS01405	313623..314390	+	768	WP_012967117.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
KVAR_RS01410	314392..315105	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
KVAR_RS01415	315277..315498	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KVAR_RS01420	315495..315863	+	369	WP_012967118.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KVAR_RS01425	316155..317471	+	1317	WP_012967119.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
KVAR_RS01430	317578..318465	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
KVAR_RS01435	318462..319307	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
KVAR_RS01440	319309..320379	+	1071	WP_008806977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	275675..321116	45441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13584.98 Da
• Isoelectric Point: 8.6410

>T24544 WP_012967118.1 NC_013850:315495-315863 [Klebsiella variicola At-22]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGMTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T24544 NC_013850:315495-315863 [Klebsiella variicola At-22]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTATGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCCGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT24544 WP_004174006.1 NC_013850:315277-315498 [Klebsiella variicola At-22]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT24544 NC_013850:315277-315498 [Klebsiella variicola At-22]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7