Detailed information of TA system TA024542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 749527..750155
• Replicon: chromosome
• Accession: NC_013849
• Organism: Ferroglobus placidus DSM 10642

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D3RX10
• Locus tag: FERP_RS04280
• Protein ID: WP_012965366.1
• Coordinates: 749527..749931 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D3RX11
• Locus tag: FERP_RS04285
• Protein ID: WP_012965367.1
• Coordinates: 749928..750155 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FERP_RS04250 (Ferp_0849)	744629..745132	+	504	WP_012965360.1	DUF429 domain-containing protein	-
FERP_RS04255 (Ferp_0850)	745124..745357	-	234	WP_012965361.1	hypothetical protein	-
FERP_RS04260 (Ferp_0851)	745533..746756	+	1224	WP_012965362.1	MBL fold metallo-hydrolase	-
FERP_RS04265 (Ferp_0852)	746753..747385	+	633	WP_012965363.1	metallophosphoesterase family protein	-
FERP_RS04270 (Ferp_0853)	747376..748212	-	837	WP_012965364.1	ABC transporter substrate-binding protein	-
FERP_RS04275 (Ferp_0854)	748209..749477	-	1269	WP_012965365.1	tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase	-
FERP_RS04280 (Ferp_0855)	749527..749931	-	405	WP_012965366.1	PIN domain-containing protein	Toxin
FERP_RS04285 (Ferp_0856)	749928..750155	-	228	WP_012965367.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FERP_RS04290 (Ferp_0857)	750249..751253	-	1005	WP_012965368.1	L-threonylcarbamoyladenylate synthase	-
FERP_RS04295 (Ferp_0858)	751250..752824	-	1575	WP_012965369.1	tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3	-
FERP_RS04300 (Ferp_0859)	752800..753873	-	1074	WP_012965370.1	CDP-alcohol phosphatidyltransferase family protein	-
FERP_RS04305 (Ferp_0860)	753854..754822	-	969	WP_012965371.1	deoxyhypusine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (3-49)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15461.83 Da
• Isoelectric Point: 6.2374

>T24542 WP_012965366.1 NC_013849:c749931-749527 [Ferroglobus placidus DSM 10642]
MRAIIDTNVLVYDTFEDSEYHKEARRLLNGLDIWMIPTIVLHEYVWVLKALNVNPNDVLYKVEEYCSHYKSKIISERLSD
VTYALETVASEGLSLSRYNDKVILSIAVREKVNLATFDEKLRKQALSKGVNVIP

Nucleotide

• Length: 405 bp

>T24542 NC_013849:c749931-749527 [Ferroglobus placidus DSM 10642]
ATGAGAGCGATAATAGATACAAACGTACTTGTGTACGATACGTTTGAAGACAGCGAATATCATAAAGAAGCGAGAAGGCT
CTTAAACGGTTTAGATATTTGGATGATACCGACGATAGTTCTTCACGAGTATGTGTGGGTTTTGAAGGCTTTGAACGTTA
ATCCTAACGATGTGCTGTACAAAGTAGAGGAATATTGTAGTCACTACAAGTCGAAGATAATTTCGGAAAGACTTTCCGAC
GTAACTTACGCTCTAGAGACGGTAGCAAGTGAGGGACTGTCTCTTTCGAGGTACAACGACAAAGTGATCTTATCAATTGC
GGTAAGGGAGAAAGTAAACCTCGCAACGTTCGATGAAAAACTCAGAAAACAAGCTCTCTCTAAAGGTGTGAATGTTATTC
CTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8852.51 Da
• Isoelectric Point: 10.5243

>AT24542 WP_012965367.1 NC_013849:c750155-749928 [Ferroglobus placidus DSM 10642]
MRVKITRNYQITIPAEVRKKMGLKIGDLLEVEYDKNKEVIIIRKVKEERRTLKVGRRLTPEDIENLMTKGLSENL

Nucleotide

• Length: 228 bp

>AT24542 NC_013849:c750155-749928 [Ferroglobus placidus DSM 10642]
ATGAGAGTGAAAATTACGAGAAATTATCAAATTACTATACCGGCGGAGGTTAGGAAGAAAATGGGATTGAAGATAGGGGA
TCTACTGGAGGTCGAGTACGATAAAAATAAAGAAGTGATAATAATAAGAAAAGTAAAAGAAGAAAGAAGAACTTTAAAAG
TTGGCAGAAGGCTTACACCGGAAGATATAGAGAATTTGATGACAAAGGGTTTAAGTGAGAACCTATGA

Structures

Toxin

• AlphaFold DB: D3RX10

Antitoxin

• AlphaFold DB: D3RX11