Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2495285..2496095 Replicon chromosome
Accession NZ_CP097419
Organism Klebsiella pneumoniae strain K229

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag M8Y66_RS12035 Protein ID WP_002887280.1
Coordinates 2495562..2496095 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M8Y66_RS12030 Protein ID WP_002887278.1
Coordinates 2495285..2495551 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y66_RS12010 (M8Y66_12010) 2491157..2491501 - 345 WP_002887266.1 cation efflux system protein CusF -
M8Y66_RS12015 (M8Y66_12015) 2491519..2492904 - 1386 WP_002887267.1 efflux transporter outer membrane subunit -
M8Y66_RS12020 (M8Y66_12020) 2493076..2493759 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
M8Y66_RS12025 (M8Y66_12025) 2493749..2495182 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
M8Y66_RS12030 (M8Y66_12030) 2495285..2495551 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M8Y66_RS12035 (M8Y66_12035) 2495562..2496095 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
M8Y66_RS12040 (M8Y66_12040) 2496143..2497264 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T245415 WP_002887280.1 NZ_CP097419:2495562-2496095 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT245415 WP_002887278.1 NZ_CP097419:2495285-2495551 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References