Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1911837..1912462 Replicon chromosome
Accession NZ_CP097419
Organism Klebsiella pneumoniae strain K229

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M8Y66_RS09245 Protein ID WP_002882817.1
Coordinates 1912079..1912462 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M8Y66_RS09240 Protein ID WP_004150355.1
Coordinates 1911837..1912079 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y66_RS09215 (M8Y66_09215) 1907828..1908427 + 600 WP_004151865.1 glucose-1-phosphatase -
M8Y66_RS09220 (M8Y66_09220) 1908421..1909281 + 861 WP_002882807.1 virulence factor BrkB family protein -
M8Y66_RS09225 (M8Y66_09225) 1909278..1909715 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M8Y66_RS09230 (M8Y66_09230) 1909760..1910701 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M8Y66_RS09235 (M8Y66_09235) 1910715..1911632 - 918 WP_002882812.1 alpha/beta hydrolase -
M8Y66_RS09240 (M8Y66_09240) 1911837..1912079 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M8Y66_RS09245 (M8Y66_09245) 1912079..1912462 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M8Y66_RS09250 (M8Y66_09250) 1912636..1913565 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M8Y66_RS09255 (M8Y66_09255) 1913562..1914197 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M8Y66_RS09260 (M8Y66_09260) 1914194..1915096 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T245412 WP_002882817.1 NZ_CP097419:1912079-1912462 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT245412 WP_004150355.1 NZ_CP097419:1911837-1912079 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References