Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5289527..5290152 Replicon chromosome
Accession NZ_CP097415
Organism Klebsiella pneumoniae strain K163

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M8Y70_RS25720 Protein ID WP_002882817.1
Coordinates 5289527..5289910 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M8Y70_RS25725 Protein ID WP_004150355.1
Coordinates 5289910..5290152 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y70_RS25705 (5286893) 5286893..5287795 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M8Y70_RS25710 (5287792) 5287792..5288427 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M8Y70_RS25715 (5288424) 5288424..5289353 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M8Y70_RS25720 (5289527) 5289527..5289910 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M8Y70_RS25725 (5289910) 5289910..5290152 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M8Y70_RS25730 (5290357) 5290357..5291274 + 918 WP_009484979.1 alpha/beta hydrolase -
M8Y70_RS25735 (5291288) 5291288..5292229 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M8Y70_RS25740 (5292274) 5292274..5292711 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M8Y70_RS25745 (5292708) 5292708..5293568 - 861 WP_004146232.1 virulence factor BrkB family protein -
M8Y70_RS25750 (5293562) 5293562..5294161 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T245406 WP_002882817.1 NZ_CP097415:c5289910-5289527 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT245406 WP_004150355.1 NZ_CP097415:c5290152-5289910 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References