Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5291053..5291678 Replicon chromosome
Accession NZ_CP097411
Organism Klebsiella pneumoniae strain K165-2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M8Y69_RS25735 Protein ID WP_002882817.1
Coordinates 5291053..5291436 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M8Y69_RS25740 Protein ID WP_004150355.1
Coordinates 5291436..5291678 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y69_RS25720 (5288419) 5288419..5289321 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M8Y69_RS25725 (5289318) 5289318..5289953 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M8Y69_RS25730 (5289950) 5289950..5290879 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M8Y69_RS25735 (5291053) 5291053..5291436 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M8Y69_RS25740 (5291436) 5291436..5291678 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M8Y69_RS25745 (5291883) 5291883..5292800 + 918 WP_009484979.1 alpha/beta hydrolase -
M8Y69_RS25750 (5292814) 5292814..5293755 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M8Y69_RS25755 (5293800) 5293800..5294237 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M8Y69_RS25760 (5294234) 5294234..5295094 - 861 WP_004146232.1 virulence factor BrkB family protein -
M8Y69_RS25765 (5295088) 5295088..5295687 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T245390 WP_002882817.1 NZ_CP097411:c5291436-5291053 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT245390 WP_004150355.1 NZ_CP097411:c5291678-5291436 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References