Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4683228..4684038 Replicon chromosome
Accession NZ_CP097411
Organism Klebsiella pneumoniae strain K165-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M8Y69_RS22900 Protein ID WP_004178461.1
Coordinates 4683228..4683761 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M8Y69_RS22905 Protein ID WP_002887278.1
Coordinates 4683772..4684038 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y69_RS22895 (4682059) 4682059..4683180 + 1122 WP_004186850.1 cupin domain-containing protein -
M8Y69_RS22900 (4683228) 4683228..4683761 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M8Y69_RS22905 (4683772) 4683772..4684038 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M8Y69_RS22910 (4684141) 4684141..4685574 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
M8Y69_RS22915 (4685564) 4685564..4686247 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M8Y69_RS22920 (4686420) 4686420..4687805 + 1386 WP_026005908.1 efflux transporter outer membrane subunit -
M8Y69_RS22925 (4687823) 4687823..4688167 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T245386 WP_004178461.1 NZ_CP097411:c4683761-4683228 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT245386 WP_002887278.1 NZ_CP097411:c4684038-4683772 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References