Detailed information of TA system TA024537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 246688..247326
• Replicon: chromosome
• Accession: NC_013791
• Organism: Bacillus pseudofirmus OF4

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: U6SMK3
• Locus tag: BPOF4_RS01305
• Protein ID: WP_012957256.1
• Coordinates: 246976..247326 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BPOF4_RS01300
• Protein ID: WP_031311629.1
• Coordinates: 246688..246972 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPOF4_RS01270	241895..242212	+	318	WP_012957249.1	DUF2325 domain-containing protein	-
BPOF4_RS01275	242236..242799	+	564	WP_012957250.1	hypothetical protein	-
BPOF4_RS01280	243087..243989	+	903	WP_041822190.1	zinc ABC transporter substrate-binding protein	-
BPOF4_RS01285	244036..244776	-	741	WP_012957252.1	rhomboid family intramembrane serine protease	-
BPOF4_RS01290	244878..245237	+	360	WP_012957253.1	holo-ACP synthase	-
BPOF4_RS01295	245476..246486	+	1011	WP_012957254.1	outer membrane lipoprotein carrier protein LolA	-
BPOF4_RS01300	246688..246972	+	285	WP_031311629.1	hypothetical protein	Antitoxin
BPOF4_RS01305	246976..247326	+	351	WP_012957256.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BPOF4_RS01310	247571..248389	+	819	WP_012957257.1	STAS domain-containing protein	-
BPOF4_RS01315	248406..248762	+	357	WP_012957258.1	STAS domain-containing protein	-
BPOF4_RS01320	248765..249178	+	414	WP_012957259.1	anti-sigma regulatory factor	-
BPOF4_RS01325	249191..250204	+	1014	WP_012957260.1	PP2C family protein-serine/threonine phosphatase	-
BPOF4_RS01330	250266..250598	+	333	WP_012957261.1	STAS domain-containing protein	-
BPOF4_RS01335	250595..251083	+	489	WP_012957262.1	anti-sigma B factor RsbW	-
BPOF4_RS01340	251043..251828	+	786	WP_012957263.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12981.03 Da
• Isoelectric Point: 6.4662

>T24537 WP_012957256.1 NC_013791:246976-247326 [Bacillus pseudofirmus OF4]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQV
RTIDKQRLTDKITHLDDDMMTRVNDALFISLGLIDF

Nucleotide

• Length: 351 bp

>T24537 NC_013791:246976-247326 [Bacillus pseudofirmus OF4]
TTGATTGTAAAAAGAGGTGACGTTTATTTTGCCGACCTTTCACCTGTTGTTGGGTCTGAGCAGGGTGGGGTAAGACCTGT
TTTAATCATCCAAAATGATATTGGGAATCGTTTTAGTCCTACTGTCATTGTGGCTGCAATCACGGCACAAATTCAAAAGG
CAAAATTGCCGACTCATGTTGAAATCAATGCAAAAAGATACGGATTTGATCGTGATTCGGTCATTTTGCTAGAACAAGTA
AGAACAATTGATAAACAGCGCCTGACCGATAAAATCACCCATCTTGATGATGATATGATGACGAGGGTTAATGATGCTTT
GTTTATTAGTTTGGGATTAATTGACTTTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10959.48 Da
• Isoelectric Point: 5.1667

>AT24537 WP_031311629.1 NC_013791:246688-246972 [Bacillus pseudofirmus OF4]
MFVSEQRTKRIVVNLPQHLLNEVDGVIQQENVNYSEFLSQATKLYLRDRKKRQIRETMQQGYMEMAKINLNLASEAFLAE
EEAENTLDRLVSGV

Nucleotide

• Length: 285 bp

>AT24537 NC_013791:246688-246972 [Bacillus pseudofirmus OF4]
ATGTTCGTGTCAGAACAGAGAACGAAACGCATTGTAGTGAATTTGCCACAACATTTGTTAAACGAGGTGGACGGTGTGAT
TCAACAAGAGAATGTAAATTACAGTGAGTTTTTATCGCAAGCAACGAAGCTATATCTTCGTGACCGAAAGAAGCGTCAAA
TTCGTGAAACGATGCAGCAAGGCTATATGGAAATGGCTAAAATTAACCTTAACCTTGCATCTGAAGCCTTTCTTGCCGAG
GAGGAAGCAGAGAACACCCTTGATCGCTTAGTAAGTGGGGTGTAG

Structures

Toxin

• AlphaFold DB: U6SMK3