Detailed information of TA system TA245351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1694163..1695858
• Replicon: chromosome
• Accession: NZ_CP097383
• Organism: Pseudomonas aeruginosa strain L00-a

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: M8G39_RS07930
• Protein ID: WP_003112901.1
• Coordinates: 1694163..1695032 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: M8G39_RS07935
• Protein ID: WP_003112902.1
• Coordinates: 1695025..1695858 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M8G39_RS07905 (M8G39_07905)	1690171..1691022	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
M8G39_RS07910 (M8G39_07910)	1691063..1692070	+	1008	WP_023097315.1	ABC transporter substrate-binding protein	-
M8G39_RS07915 (M8G39_07915)	1692067..1692843	+	777	WP_003112898.1	ABC transporter permease	-
M8G39_RS07920 (M8G39_07920)	1692849..1693610	+	762	WP_003112899.1	SDR family oxidoreductase	-
M8G39_RS07925 (M8G39_07925)	1693624..1694154	+	531	WP_003112900.1	cupin domain-containing protein	-
M8G39_RS07930 (M8G39_07930)	1694163..1695032	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
M8G39_RS07935 (M8G39_07935)	1695025..1695858	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
M8G39_RS07940 (M8G39_07940)	1695845..1696642	+	798	WP_023111480.1	SDR family oxidoreductase	-
M8G39_RS07945 (M8G39_07945)	1696635..1698317	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
M8G39_RS07950 (M8G39_07950)	1698328..1699131	+	804	WP_003130116.1	aspartate dehydrogenase	-
M8G39_RS07955 (M8G39_07955)	1699143..1700627	+	1485	WP_023102925.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T245351 WP_003112901.1 NZ_CP097383:1694163-1695032 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT245351 WP_003112902.1 NZ_CP097383:1695025-1695858 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1