Detailed information of TA system TA024535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-VapB
• Location: 2671837..2672399
• Replicon: chromosome
• Accession: NC_013757
• Organism: Geodermatophilus obscurus DSM 43160

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D2S5E6
• Locus tag: GOBS_RS12550
• Protein ID: WP_012948661.1
• Coordinates: 2671837..2672166 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GOBS_RS12555
• Protein ID: WP_041241469.1
• Coordinates: 2672163..2672399 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOBS_RS12530	2667723..2668082	-	360	WP_012948658.1	hypothetical protein	-
GOBS_RS12535	2668303..2669568	-	1266	WP_012948659.1	FAD-binding protein	-
GOBS_RS12540	2670367..2670825	-	459	WP_012948660.1	RpiB/LacA/LacB family sugar-phosphate isomerase	-
GOBS_RS12545	2671166..2671426	-	261	WP_041241468.1	DUF2252 family protein	-
GOBS_RS12550	2671837..2672166	-	330	WP_012948661.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GOBS_RS12555	2672163..2672399	-	237	WP_041241469.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GOBS_RS12560	2673283..2674330	-	1048	Protein_2532	methyltransferase domain-containing protein	-
GOBS_RS12565	2674458..2677331	-	2874	WP_012948663.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2665882..2683571	17689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-106)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (9-46)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11846.83 Da
• Isoelectric Point: 6.8831

>T24535 WP_012948661.1 NC_013757:c2672166-2671837 [Geodermatophilus obscurus DSM 43160]
VTTLPARGEVWWCEMAEIGRRPVVVLSRDAVIPRHRRALVAPCTTTIRGLVSEVGLEPGEDPVPRRCVVNMDSVESVSVA
VLVERLGRLADTRMRQICGALAVAVDCAD

Nucleotide

• Length: 330 bp

>T24535 NC_013757:c2672166-2671837 [Geodermatophilus obscurus DSM 43160]
GTGACCACGCTGCCCGCGCGCGGTGAGGTCTGGTGGTGCGAGATGGCCGAGATCGGGCGCCGCCCGGTCGTCGTCCTCTC
CCGCGACGCAGTGATCCCCCGACACCGGCGCGCACTCGTCGCACCGTGCACCACCACGATCCGCGGCCTCGTCAGCGAAG
TCGGGCTCGAGCCCGGCGAGGACCCGGTACCCCGCCGCTGCGTGGTCAACATGGACTCGGTCGAGAGCGTCTCCGTCGCG
GTCCTCGTCGAGCGGCTCGGACGGTTGGCCGACACGCGCATGCGGCAGATCTGCGGAGCCCTCGCCGTCGCGGTCGACTG
CGCGGACTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8620.42 Da
• Isoelectric Point: 4.6135

>AT24535 WP_041241469.1 NC_013757:c2672399-2672163 [Geodermatophilus obscurus DSM 43160]
MSRTRLSTTVDDALLQAARRVRSGNTDAAMIDEALRALVARHRSAEVEASYTAYDEHPLDEPDEWGDLASFREAAGRS

Nucleotide

• Length: 237 bp

>AT24535 NC_013757:c2672399-2672163 [Geodermatophilus obscurus DSM 43160]
ATGAGTAGAACACGGCTGAGCACCACCGTCGACGACGCTCTCCTCCAGGCCGCTCGACGTGTGCGATCCGGGAACACCGA
CGCCGCCATGATCGATGAGGCGCTGCGCGCGCTGGTCGCGCGCCACCGGTCGGCCGAAGTCGAGGCGAGCTACACCGCCT
ACGACGAACACCCCCTCGACGAACCAGACGAGTGGGGTGACCTGGCGTCGTTCCGCGAAGCCGCCGGACGGTCGTGA

Structures

Toxin

• AlphaFold DB: D2S5E6