Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4034624..4035200 Replicon chromosome
Accession NZ_CP097370
Organism Escherichia coli strain AGR5151

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M7748_RS19430 Protein ID WP_001297643.1
Coordinates 4034913..4035200 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M7748_RS19425 Protein ID WP_000063156.1
Coordinates 4034624..4034926 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7748_RS19410 (4031220) 4031220..4033370 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M7748_RS19415 (4033420) 4033420..4033623 + 204 WP_000467859.1 YbdD/YjiX family protein -
M7748_RS19420 (4033634) 4033634..4034590 + 957 WP_001297640.1 GTPase -
M7748_RS19425 (4034624) 4034624..4034926 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M7748_RS19430 (4034913) 4034913..4035200 - 288 WP_001297643.1 BrnT family toxin Toxin
M7748_RS19440 (4036153) 4036153..4036248 + 96 Protein_3811 dienelactone hydrolase family protein -
M7748_RS19445 (4036394) 4036394..4037626 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
M7748_RS19450 (4037667) 4037667..4038947 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4035399..4035692 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T245330 WP_001297643.1 NZ_CP097370:c4035200-4034913 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT245330 WP_000063156.1 NZ_CP097370:c4034926-4034624 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References