Detailed information of TA system TA024531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 5140344..5140975
• Replicon: chromosome
• Accession: NC_013739
• Organism: Conexibacter woesei DSM 14684

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D3FB40
• Locus tag: CWOE_RS24205
• Protein ID: WP_012936283.1
• Coordinates: 5140592..5140975 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D3FB39
• Locus tag: CWOE_RS24200
• Protein ID: WP_012936282.1
• Coordinates: 5140344..5140595 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWOE_RS24180	5136485..5137312	-	828	WP_148261139.1	DeoR/GlpR family DNA-binding transcription regulator	-
CWOE_RS24185	5137460..5138203	+	744	WP_012936279.1	(Fe-S)-binding protein	-
CWOE_RS24190	5138200..5139639	+	1440	WP_012936280.1	iron-sulfur cluster-binding protein	-
CWOE_RS24195	5139636..5140292	+	657	WP_012936281.1	lactate utilization protein C	-
CWOE_RS24200	5140344..5140595	+	252	WP_012936282.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CWOE_RS24205	5140592..5140975	+	384	WP_012936283.1	type II toxin-antitoxin system VapC family toxin	Toxin
CWOE_RS24210	5141055..5141579	+	525	WP_012936284.1	PaaI family thioesterase	-
CWOE_RS24215	5141606..5142070	-	465	WP_012936285.1	transcription elongation factor GreA	-
CWOE_RS24220	5142181..5144361	+	2181	WP_012936286.1	DEAD/DEAH box helicase	-
CWOE_RS31365	5144406..5145101	+	696	WP_049793390.1	hypothetical protein	-
CWOE_RS24230	5145098..5145640	+	543	WP_012936288.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 7.1689

>T24531 WP_012936283.1 NC_013739:5140592-5140975 [Conexibacter woesei DSM 14684]
MSRALADTSVFVAQESGRTLGELPDRIAVSVVTAAELELGVLRARDAATRARRLNTLARVRSAYPLLPIDAETASCFAGI
ADEELGQGRRLRRHDVWIAATALRHGVPVVTQDADFTAFDLVTVLRV

Nucleotide

• Length: 384 bp

>T24531 NC_013739:5140592-5140975 [Conexibacter woesei DSM 14684]
ATGAGCCGCGCGCTCGCCGACACGTCGGTCTTCGTTGCGCAGGAGTCCGGCCGCACGCTCGGGGAGCTGCCCGACCGCAT
CGCCGTCTCGGTCGTCACCGCAGCCGAGCTGGAGCTGGGCGTGCTGCGAGCGCGCGATGCCGCCACCCGCGCACGCCGCC
TCAACACGCTGGCCCGCGTGCGGAGCGCCTACCCGCTGCTGCCGATCGACGCGGAGACCGCGTCGTGCTTCGCCGGGATC
GCAGACGAGGAGCTGGGTCAGGGCAGGAGACTGCGCCGCCACGACGTCTGGATCGCCGCGACCGCACTCCGGCACGGCGT
GCCGGTCGTGACGCAGGACGCCGACTTCACGGCGTTCGACCTCGTGACGGTTCTGCGCGTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9166.41 Da
• Isoelectric Point: 4.8552

>AT24531 WP_012936282.1 NC_013739:5140344-5140595 [Conexibacter woesei DSM 14684]
MAKEVSVRELRNHTADVVAAVRGGETMTLTVNRERVADIVPHVERRDPWVPASVLREIVREAPADRGLLADLADVRGALL
DDE

Nucleotide

• Length: 252 bp

>AT24531 NC_013739:5140344-5140595 [Conexibacter woesei DSM 14684]
ATGGCGAAAGAGGTCTCCGTGCGCGAGCTGCGCAACCACACCGCGGACGTCGTCGCCGCCGTCCGCGGCGGCGAGACGAT
GACGCTGACGGTCAACCGCGAGCGGGTCGCGGACATCGTCCCGCACGTCGAGCGGCGCGACCCGTGGGTGCCGGCGTCGG
TGCTGCGCGAGATCGTGCGAGAGGCCCCGGCCGACCGCGGGCTGCTCGCCGACCTCGCCGACGTCCGCGGCGCGCTCCTC
GACGACGAATGA

Structures

Toxin

• AlphaFold DB: D3FB40

Antitoxin

• AlphaFold DB: D3FB39