Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4141575..4142151 Replicon chromosome
Accession NZ_CP097360
Organism Escherichia coli strain AGR4587

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M7749_RS20205 Protein ID WP_001297643.1
Coordinates 4141864..4142151 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M7749_RS20200 Protein ID WP_000063156.1
Coordinates 4141575..4141877 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7749_RS20185 (4138171) 4138171..4140321 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M7749_RS20190 (4140371) 4140371..4140574 + 204 WP_000467859.1 YbdD/YjiX family protein -
M7749_RS20195 (4140585) 4140585..4141541 + 957 WP_001297640.1 GTPase -
M7749_RS20200 (4141575) 4141575..4141877 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M7749_RS20205 (4141864) 4141864..4142151 - 288 WP_001297643.1 BrnT family toxin Toxin
M7749_RS20210 (4142350) 4142350..4143047 - 698 Protein_3961 IS1 family transposase -
M7749_RS20215 (4143104) 4143104..4143199 + 96 Protein_3962 dienelactone hydrolase family protein -
M7749_RS20220 (4143345) 4143345..4144577 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
M7749_RS20225 (4144618) 4144618..4145898 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T245283 WP_001297643.1 NZ_CP097360:c4142151-4141864 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT245283 WP_000063156.1 NZ_CP097360:c4141877-4141575 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References