Detailed information of TA system TA024527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3099060..3099601
• Replicon: chromosome
• Accession: NC_013729
• Organism: Kribbella flavida DSM 17836

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D2Q0I9
• Locus tag: KFLA_RS14440
• Protein ID: WP_012920537.1
• Coordinates: 3099060..3099365 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D2Q0J0
• Locus tag: KFLA_RS14445
• Protein ID: WP_012920538.1
• Coordinates: 3099365..3099601 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFLA_RS14425	3095716..3096135	-	420	WP_012920534.1	VOC family protein	-
KFLA_RS14430	3096132..3096986	-	855	WP_041289326.1	helix-turn-helix domain-containing protein	-
KFLA_RS14435	3097137..3098987	+	1851	WP_012920536.1	dihydroxy-acid dehydratase	-
KFLA_RS14440	3099060..3099365	-	306	WP_012920537.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KFLA_RS14445	3099365..3099601	-	237	WP_012920538.1	ribbon-helix-helix domain-containing protein	Antitoxin
KFLA_RS14450	3099652..3100320	-	669	WP_012920539.1	LON peptidase substrate-binding domain-containing protein	-
KFLA_RS14455	3100538..3101863	+	1326	WP_012920540.1	histidinol dehydrogenase	-
KFLA_RS14460	3101863..3102984	+	1122	WP_012920541.1	histidinol-phosphate transaminase	-
KFLA_RS14465	3103052..3103651	+	600	WP_012920542.1	imidazoleglycerol-phosphate dehydratase HisB	-
KFLA_RS14470	3103648..3104289	+	642	WP_012920543.1	imidazole glycerol phosphate synthase subunit HisH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11110.02 Da
• Isoelectric Point: 8.0510

>T24527 WP_012920537.1 NC_013729:c3099365-3099060 [Kribbella flavida DSM 17836]
MRPIHIARLDKPRPVVVLTRELIRPRLTNVTVAPITSTVRGLSTEVLVGPQNGLDHPSAISCDNIQTIPKLQLGRLIGYL
LPDQEPALTEAVTLAFDLDLR

Nucleotide

• Length: 306 bp

>T24527 NC_013729:c3099365-3099060 [Kribbella flavida DSM 17836]
ATGCGGCCCATCCACATCGCCCGGCTGGACAAACCCCGCCCGGTGGTGGTGCTCACCCGCGAGCTGATTCGCCCCCGGCT
GACGAACGTCACCGTCGCCCCGATCACCAGCACCGTGCGCGGCCTGTCCACCGAGGTCCTGGTCGGCCCGCAGAACGGTC
TCGACCACCCGAGCGCGATCAGCTGCGACAACATCCAGACGATCCCGAAGCTCCAGCTCGGCCGCCTGATCGGCTACCTG
CTGCCCGACCAGGAACCAGCCCTCACCGAAGCCGTCACGCTGGCCTTCGACCTCGATCTCCGGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8390.47 Da
• Isoelectric Point: 4.4419

>AT24527 WP_012920538.1 NC_013729:c3099601-3099365 [Kribbella flavida DSM 17836]
MSKQITVRLPDELVEFMDQLVDANKASSRASVVARAMERERRRELAARDLALLAAHGEPDDLDGLAAAAGGTVLSDLD

Nucleotide

• Length: 237 bp

>AT24527 NC_013729:c3099601-3099365 [Kribbella flavida DSM 17836]
ATGAGCAAGCAGATCACTGTGCGGTTGCCGGACGAGCTGGTGGAGTTCATGGACCAGCTCGTCGACGCCAACAAGGCATC
CTCCCGGGCCTCCGTGGTGGCCCGGGCGATGGAGCGTGAGCGGCGGCGGGAGCTGGCCGCGCGGGATCTCGCGTTGCTGG
CCGCGCACGGCGAGCCCGACGATCTCGACGGGCTCGCCGCGGCCGCCGGCGGTACCGTGCTGTCCGACCTCGACTGA

Structures

Toxin

• AlphaFold DB: D2Q0I9

Antitoxin

• AlphaFold DB: D2Q0J0