Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3918893..3919530 Replicon chromosome
Accession NZ_CP097359
Organism Bacillus velezensis strain H208

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M5C55_RS19360 Protein ID WP_003156187.1
Coordinates 3919180..3919530 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M5C55_RS19355 Protein ID WP_003156188.1
Coordinates 3918893..3919174 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5C55_RS19335 (M5C55_19335) 3915258..3915857 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
M5C55_RS19340 (M5C55_19340) 3915950..3916315 + 366 WP_007609580.1 holo-ACP synthase -
M5C55_RS19345 (M5C55_19345) 3916480..3917487 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
M5C55_RS19350 (M5C55_19350) 3917604..3918773 + 1170 WP_032873001.1 alanine racemase -
M5C55_RS19355 (M5C55_19355) 3918893..3919174 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M5C55_RS19360 (M5C55_19360) 3919180..3919530 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M5C55_RS19365 (M5C55_19365) 3919648..3920469 + 822 WP_003156182.1 STAS domain-containing protein -
M5C55_RS19370 (M5C55_19370) 3920474..3920839 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M5C55_RS19375 (M5C55_19375) 3920842..3921243 + 402 WP_003156178.1 anti-sigma regulatory factor -
M5C55_RS19380 (M5C55_19380) 3921255..3922262 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
M5C55_RS19385 (M5C55_19385) 3922326..3922655 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M5C55_RS19390 (M5C55_19390) 3922652..3923134 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M5C55_RS19395 (M5C55_19395) 3923100..3923888 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
M5C55_RS19400 (M5C55_19400) 3923888..3924490 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245264 WP_003156187.1 NZ_CP097359:3919180-3919530 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245264 WP_003156188.1 NZ_CP097359:3918893-3919174 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References