Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 740341..741258 Replicon chromosome
Accession NZ_CP097359
Organism Bacillus velezensis strain H208

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag M5C55_RS03890 Protein ID WP_007407256.1
Coordinates 740512..741258 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag M5C55_RS03885 Protein ID WP_003154807.1
Coordinates 740341..740511 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5C55_RS03845 (M5C55_03845) 735574..737196 + 1623 WP_032874601.1 pyocin knob domain-containing protein -
M5C55_RS03850 (M5C55_03850) 737209..737580 + 372 WP_032874603.1 XkdW family protein -
M5C55_RS03855 (M5C55_03855) 737585..737782 + 198 WP_032874605.1 XkdX family protein -
M5C55_RS03860 (M5C55_03860) 737839..738600 + 762 WP_032874607.1 hypothetical protein -
M5C55_RS03865 (M5C55_03865) 738652..738915 + 264 WP_003154815.1 hemolysin XhlA family protein -
M5C55_RS03870 (M5C55_03870) 738929..739192 + 264 WP_003154813.1 phage holin -
M5C55_RS03875 (M5C55_03875) 739206..740084 + 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
M5C55_RS03880 (M5C55_03880) 740119..740244 - 126 WP_003154809.1 hypothetical protein -
M5C55_RS03885 (M5C55_03885) 740341..740511 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
M5C55_RS03890 (M5C55_03890) 740512..741258 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
M5C55_RS03895 (M5C55_03895) 741363..742361 - 999 WP_032874611.1 inorganic phosphate transporter -
M5C55_RS03900 (M5C55_03900) 742374..742991 - 618 WP_032874614.1 DUF47 domain-containing protein -
M5C55_RS03905 (M5C55_03905) 743277..744593 - 1317 WP_032874616.1 amino acid permease -
M5C55_RS03910 (M5C55_03910) 744915..745865 + 951 WP_032874618.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T245263 WP_007407256.1 NZ_CP097359:c741258-740512 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT245263 WP_003154807.1 NZ_CP097359:c740511-740341 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References