Detailed information of TA system TA024526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 1105026..1105708
• Replicon: chromosome
• Accession: NC_013729
• Organism: Kribbella flavida DSM 17836

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFLA_RS05110
• Protein ID: WP_012918699.1
• Coordinates: 1105304..1105708 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D2Q1F4
• Locus tag: KFLA_RS05105
• Protein ID: WP_012918698.1
• Coordinates: 1105026..1105307 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFLA_RS05085	1100251..1100943	-	693	WP_083792959.1	lysozyme	-
KFLA_RS05090	1101217..1102455	-	1239	WP_012918695.1	neutral zinc metallopeptidase	-
KFLA_RS05095	1102564..1104006	+	1443	WP_012918696.1	cysteine--tRNA ligase	-
KFLA_RS05100	1104011..1104973	+	963	WP_012918697.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
KFLA_RS05105	1105026..1105307	+	282	WP_012918698.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KFLA_RS05110	1105304..1105708	+	405	WP_012918699.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFLA_RS05115	1105779..1106669	-	891	WP_012918700.1	S1 family peptidase	-
KFLA_RS05120	1106784..1108436	-	1653	WP_012918701.1	alkaline phosphatase D family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-48)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14702.98 Da
• Isoelectric Point: 5.7514

>T24526 WP_012918699.1 NC_013729:1105304-1105708 [Kribbella flavida DSM 17836]
VIYLDACALLKFIKPEKESAALRKWREALPPDTELLTSELAKLEITRTLLRAGVDHQQVPYFTGQAVRGIYVVDLSSTVL
ARAMAYRTARLGSLDAIHLASAEPFRADLEQFVTYDKELSGAAAELGFPVLAPA

Nucleotide

• Length: 405 bp

>T24526 NC_013729:1105304-1105708 [Kribbella flavida DSM 17836]
GTGATCTACCTGGACGCTTGTGCGCTGCTGAAGTTCATCAAGCCGGAGAAGGAGTCGGCCGCGTTGCGCAAGTGGCGCGA
AGCGCTGCCGCCCGACACGGAACTGCTCACGAGCGAGCTGGCCAAGTTGGAGATCACCCGAACGTTGCTGCGGGCCGGCG
TCGACCATCAGCAGGTGCCGTACTTCACCGGCCAAGCGGTCCGCGGGATCTATGTCGTCGACCTCAGCTCGACCGTGCTC
GCTCGGGCGATGGCTTACCGCACGGCGCGGCTCGGTTCTCTCGACGCCATCCATCTCGCCAGTGCGGAACCGTTCCGTGC
TGACCTGGAGCAATTCGTCACGTACGACAAGGAGTTGAGCGGAGCTGCTGCCGAGCTGGGATTTCCGGTCCTCGCGCCGG
CCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10296.77 Da
• Isoelectric Point: 4.6794

>AT24526 WP_012918698.1 NC_013729:1105026-1105307 [Kribbella flavida DSM 17836]
MVTIGLRELNQNPSKAVARVRAGETIVVTDRGRPVLRLVPEQEQPATLQHMIDSGEVAPPAEFGMPDLALELAPEVESLS
DLLISDRDKERRR

Nucleotide

• Length: 282 bp

>AT24526 NC_013729:1105026-1105307 [Kribbella flavida DSM 17836]
ATGGTGACCATCGGGCTGCGCGAACTGAATCAGAACCCGTCGAAGGCGGTGGCCCGCGTCCGGGCCGGCGAGACGATCGT
GGTCACCGACCGCGGACGGCCGGTGCTCCGGCTCGTTCCCGAGCAGGAACAGCCCGCAACGCTGCAGCACATGATCGACT
CCGGCGAAGTGGCTCCACCGGCTGAGTTCGGCATGCCGGATCTGGCGCTGGAGCTGGCACCGGAGGTCGAAAGCCTTTCC
GACCTGCTGATCTCGGACCGCGACAAGGAGCGGCGTCGGTGA

Structures

Antitoxin

• AlphaFold DB: D2Q1F4