Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93654..95277 Replicon plasmid p1_va18651
Accession NZ_CP097343
Organism Enterobacter hormaechei subsp. xiangfangensis strain va18651

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag M5K26_RS23365 Protein ID WP_000088044.1
Coordinates 93952..95277 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag M5K26_RS23360 Protein ID WP_012477377.1
Coordinates 93654..93947 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5K26_RS23340 (M5K26_23315) 89453..89845 + 393 WP_000341066.1 hypothetical protein -
M5K26_RS23345 (M5K26_23320) 90504..91160 + 657 WP_000462754.1 trypsin-like serine protease -
M5K26_RS23350 (M5K26_23325) 91363..91860 + 498 WP_000340139.1 membrane protein -
M5K26_RS23355 (M5K26_23330) 91865..93253 + 1389 WP_012695445.1 hypothetical protein -
M5K26_RS23360 (M5K26_23335) 93654..93947 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
M5K26_RS23365 (M5K26_23340) 93952..95277 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
M5K26_RS23370 (M5K26_23345) 95338..95544 + 207 WP_000134171.1 hypothetical protein -
M5K26_RS23375 (M5K26_23350) 95645..96055 + 411 WP_000985909.1 hypothetical protein -
M5K26_RS23380 (M5K26_23355) 96068..96592 + 525 WP_001805097.1 hypothetical protein -
M5K26_RS23385 (M5K26_23360) 96774..97778 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
M5K26_RS23390 (M5K26_23365) 97869..98303 - 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / catA2 / tet(D) / aadA2 / qacE / sul1 / qnrA1 / dfrA19 / aph(3'')-Ib / aph(6)-Id / mcr-9 / aac(6')-IIc / ere(A) / sul2 / blaSHV-12 - 1..307415 307415
- flank IS/Tn - - 96774..97778 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T245252 WP_000088044.1 NZ_CP097343:93952-95277 [Enterobacter hormaechei subsp. xiangfangensis]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT245252 WP_012477377.1 NZ_CP097343:93654-93947 [Enterobacter hormaechei subsp. xiangfangensis]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References