Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 4784..5306 Replicon plasmid pCROD2
Accession NC_013718
Organism Citrobacter rodentium ICC168

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag ROD_RS25310 Protein ID WP_000220561.1
Coordinates 4784..5065 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag ROD_RS25315 Protein ID WP_000121743.1
Coordinates 5055..5306 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ROD_RS25270 53..400 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
ROD_RS25275 397..645 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
ROD_RS25280 951..1301 - 351 WP_000854261.1 hypothetical protein -
ROD_RS25285 1393..1692 - 300 WP_000650311.1 hypothetical protein -
ROD_RS27235 2146..2373 - 228 WP_000801440.1 hypothetical protein -
ROD_RS25290 2360..2611 - 252 WP_001230707.1 hypothetical protein -
ROD_RS25295 2608..3150 - 543 WP_012908950.1 hypothetical protein -
ROD_RS25300 3255..3770 - 516 WP_001025397.1 J domain-containing protein -
ROD_RS25305 4410..4760 - 351 WP_000312755.1 hypothetical protein -
ROD_RS25310 4784..5065 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ROD_RS25315 5055..5306 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
ROD_RS25320 6671..7678 + 1008 WP_000243325.1 replication initiation protein -
ROD_RS25325 7744..8148 + 405 WP_000654312.1 hypothetical protein -
ROD_RS25330 8284..8811 + 528 WP_000577047.1 DUF2726 domain-containing protein -
ROD_RS25340 9383..9634 - 252 WP_000905714.1 hypothetical protein -
ROD_RS25345 9671..9973 - 303 WP_001062963.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..39265 39265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T24524 WP_000220561.1 NC_013718:c5065-4784 [Citrobacter rodentium ICC168]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T24524 NC_013718:c5065-4784 [Citrobacter rodentium ICC168]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTTGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT24524 WP_000121743.1 NC_013718:c5306-5055 [Citrobacter rodentium ICC168]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT24524 NC_013718:c5306-5055 [Citrobacter rodentium ICC168]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References