Detailed information of TA system TA024523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 2146..2611
• Replicon: plasmid pCROD2
• Accession: NC_013718
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: ROD_RS25290
• Protein ID: WP_001230707.1
• Coordinates: 2360..2611 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: ROD_RS27235
• Protein ID: WP_000801440.1
• Coordinates: 2146..2373 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS25270	53..400	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
ROD_RS25275	397..645	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
ROD_RS25280	951..1301	-	351	WP_000854261.1	hypothetical protein	-
ROD_RS25285	1393..1692	-	300	WP_000650311.1	hypothetical protein	-
ROD_RS27235	2146..2373	-	228	WP_000801440.1	hypothetical protein	Antitoxin
ROD_RS25290	2360..2611	-	252	WP_001230707.1	hypothetical protein	Toxin
ROD_RS25295	2608..3150	-	543	WP_012908950.1	hypothetical protein	-
ROD_RS25300	3255..3770	-	516	WP_001025397.1	J domain-containing protein	-
ROD_RS25305	4410..4760	-	351	WP_000312755.1	hypothetical protein	-
ROD_RS25310	4784..5065	-	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ROD_RS25315	5055..5306	-	252	WP_000121743.1	plasmid stabilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39265	39265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-62)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-72)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T24523 WP_001230707.1 NC_013718:c2611-2360 [Citrobacter rodentium ICC168]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T24523 NC_013718:c2611-2360 [Citrobacter rodentium ICC168]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT24523 WP_000801440.1 NC_013718:c2373-2146 [Citrobacter rodentium ICC168]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT24523 NC_013718:c2373-2146 [Citrobacter rodentium ICC168]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAGAAAGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAGGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9