Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 15022..15611 Replicon chromosome
Accession NZ_CP097339
Organism Mannheimia haemolytica strain H-5106

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag M3710_RS00100 Protein ID WP_006248516.1
Coordinates 15022..15354 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag M3710_RS00105 Protein ID WP_006248517.1
Coordinates 15351..15611 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3710_RS00060 (M3710_00060) 10217..10837 - 621 WP_006248508.1 dTMP kinase -
M3710_RS00065 (M3710_00065) 10848..11882 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
M3710_RS00085 (M3710_00085) 12462..12989 + 528 WP_006248512.1 inorganic diphosphatase -
M3710_RS00090 (M3710_00090) 13045..13800 + 756 WP_006248513.1 M48 family metallopeptidase -
M3710_RS00095 (M3710_00095) 13864..14895 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
M3710_RS00100 (M3710_00100) 15022..15354 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M3710_RS00105 (M3710_00105) 15351..15611 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M3710_RS00110 (M3710_00110) 15681..16349 - 669 WP_006248518.1 phosphoglycolate phosphatase -
M3710_RS00115 (M3710_00115) 16436..17233 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
M3710_RS00120 (M3710_00120) 17241..18029 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
M3710_RS00125 (M3710_00125) 18031..18675 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
M3710_RS00130 (M3710_00130) 19287..20432 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T245222 WP_006248516.1 NZ_CP097339:c15354-15022 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT245222 WP_006248517.1 NZ_CP097339:c15611-15351 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References