Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 53..645 Replicon plasmid pCROD2
Accession NC_013718
Organism Citrobacter rodentium ICC168

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag ROD_RS25275 Protein ID WP_000520549.1
Coordinates 397..645 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag ROD_RS25270 Protein ID WP_000681613.1
Coordinates 53..400 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ROD_RS25270 53..400 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ROD_RS25275 397..645 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
ROD_RS25280 951..1301 - 351 WP_000854261.1 hypothetical protein -
ROD_RS25285 1393..1692 - 300 WP_000650311.1 hypothetical protein -
ROD_RS27235 2146..2373 - 228 WP_000801440.1 hypothetical protein -
ROD_RS25290 2360..2611 - 252 WP_001230707.1 hypothetical protein -
ROD_RS25295 2608..3150 - 543 WP_012908950.1 hypothetical protein -
ROD_RS25300 3255..3770 - 516 WP_001025397.1 J domain-containing protein -
ROD_RS25305 4410..4760 - 351 WP_000312755.1 hypothetical protein -
ROD_RS25310 4784..5065 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin -
ROD_RS25315 5055..5306 - 252 WP_000121743.1 plasmid stabilization protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..39265 39265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T24522 WP_000520549.1 NC_013718:c645-397 [Citrobacter rodentium ICC168]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T24522 NC_013718:c645-397 [Citrobacter rodentium ICC168]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT24522 WP_000681613.1 NC_013718:c400-53 [Citrobacter rodentium ICC168]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT24522 NC_013718:c400-53 [Citrobacter rodentium ICC168]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGACTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References