Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 1650328..1650850 Replicon chromosome
Accession NZ_CP097337
Organism Mannheimia haemolytica strain NCTC 9712

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag M3706_RS08330 Protein ID WP_006249269.1
Coordinates 1650554..1650850 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A378NDF7
Locus tag M3706_RS08325 Protein ID WP_006250574.1
Coordinates 1650328..1650570 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3706_RS08300 (M3706_08300) 1646178..1647255 - 1078 Protein_1622 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
M3706_RS08305 (M3706_08305) 1647429..1648070 - 642 WP_006250576.1 orotate phosphoribosyltransferase -
M3706_RS08310 (M3706_08310) 1648193..1649035 - 843 WP_006250575.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
M3706_RS08315 (M3706_08315) 1649095..1649670 + 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
M3706_RS08320 (M3706_08320) 1649648..1650316 - 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
M3706_RS08325 (M3706_08325) 1650328..1650570 - 243 WP_006250574.1 hypothetical protein Antitoxin
M3706_RS08330 (M3706_08330) 1650554..1650850 - 297 WP_006249269.1 BrnT family toxin Toxin
M3706_RS08335 (M3706_08335) 1650977..1653889 - 2913 WP_006250573.1 RNA polymerase-associated protein RapA -
M3706_RS08340 (M3706_08340) 1654050..1654349 - 300 WP_006249265.1 hypothetical protein -
M3706_RS08345 (M3706_08345) 1654470..1655795 - 1326 WP_020831251.1 anti-phage deoxyguanosine triphosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T245210 WP_006249269.1 NZ_CP097337:c1650850-1650554 [Mannheimia haemolytica]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9320.71 Da        Isoelectric Point: 7.1665

>AT245210 WP_006250574.1 NZ_CP097337:c1650570-1650328 [Mannheimia haemolytica]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALSKMWIAEKLPVQSPR

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A378NDF7

References