Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 111767..112356 Replicon chromosome
Accession NZ_CP097337
Organism Mannheimia haemolytica strain NCTC 9712

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag M3706_RS00585 Protein ID WP_006248516.1
Coordinates 111767..112099 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag M3706_RS00590 Protein ID WP_006248517.1
Coordinates 112096..112356 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3706_RS00545 (M3706_00545) 106962..107582 - 621 WP_006248508.1 dTMP kinase -
M3706_RS00550 (M3706_00550) 107593..108627 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
M3706_RS00570 (M3706_00570) 109207..109734 + 528 WP_006248512.1 inorganic diphosphatase -
M3706_RS00575 (M3706_00575) 109790..110545 + 756 WP_006248513.1 M48 family metallopeptidase -
M3706_RS00580 (M3706_00580) 110609..111640 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
M3706_RS00585 (M3706_00585) 111767..112099 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M3706_RS00590 (M3706_00590) 112096..112356 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M3706_RS00595 (M3706_00595) 112426..113094 - 669 WP_006248518.1 phosphoglycolate phosphatase -
M3706_RS00600 (M3706_00600) 113181..113978 - 798 WP_006251103.1 prolipoprotein diacylglyceryl transferase -
M3706_RS00605 (M3706_00605) 113986..114774 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
M3706_RS00610 (M3706_00610) 114776..115420 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
M3706_RS00615 (M3706_00615) 116032..117177 + 1146 WP_006251102.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T245205 WP_006248516.1 NZ_CP097337:c112099-111767 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT245205 WP_006248517.1 NZ_CP097337:c112356-112096 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References