Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 249965..250554 Replicon chromosome
Accession NZ_CP097336
Organism Mannheimia haemolytica strain NCTC 10636

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag M3705_RS01365 Protein ID WP_249763812.1
Coordinates 249965..250297 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag M3705_RS01370 Protein ID WP_006248517.1
Coordinates 250294..250554 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3705_RS01325 (M3705_01325) 245161..245781 - 621 WP_006248508.1 dTMP kinase -
M3705_RS01330 (M3705_01330) 245792..246826 - 1035 WP_249763811.1 endolytic transglycosylase MltG -
M3705_RS01350 (M3705_01350) 247405..247932 + 528 WP_006248512.1 inorganic diphosphatase -
M3705_RS01355 (M3705_01355) 247988..248743 + 756 WP_006248513.1 M48 family metallopeptidase -
M3705_RS01360 (M3705_01360) 248807..249838 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
M3705_RS01365 (M3705_01365) 249965..250297 - 333 WP_249763812.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M3705_RS01370 (M3705_01370) 250294..250554 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M3705_RS01375 (M3705_01375) 250624..251292 - 669 WP_006248518.1 phosphoglycolate phosphatase -
M3705_RS01380 (M3705_01380) 251379..252176 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
M3705_RS01385 (M3705_01385) 252184..252972 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
M3705_RS01390 (M3705_01390) 252974..253618 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
M3705_RS01395 (M3705_01395) 254230..255375 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.94 Da        Isoelectric Point: 6.4597

>T245195 WP_249763812.1 NZ_CP097336:c250297-249965 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVAATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT245195 WP_006248517.1 NZ_CP097336:c250554-250294 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References