Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 305711..306300 Replicon chromosome
Accession NZ_CP097334
Organism Mannheimia haemolytica strain 183

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag M3707_RS01570 Protein ID WP_006248516.1
Coordinates 305711..306043 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag M3707_RS01575 Protein ID WP_006248517.1
Coordinates 306040..306300 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3707_RS01530 (M3707_01530) 300906..301526 - 621 WP_006248508.1 dTMP kinase -
M3707_RS01535 (M3707_01535) 301537..302571 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
M3707_RS01555 (M3707_01555) 303151..303678 + 528 WP_006248512.1 inorganic diphosphatase -
M3707_RS01560 (M3707_01560) 303734..304489 + 756 WP_006248513.1 M48 family metallopeptidase -
M3707_RS01565 (M3707_01565) 304553..305584 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
M3707_RS01570 (M3707_01570) 305711..306043 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M3707_RS01575 (M3707_01575) 306040..306300 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M3707_RS01580 (M3707_01580) 306370..307038 - 669 WP_006248518.1 phosphoglycolate phosphatase -
M3707_RS01585 (M3707_01585) 307125..307922 - 798 WP_006248519.1 prolipoprotein diacylglyceryl transferase -
M3707_RS01590 (M3707_01590) 307930..308718 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
M3707_RS01595 (M3707_01595) 308720..309364 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
M3707_RS01600 (M3707_01600) 309976..311121 + 1146 WP_006248526.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T245182 WP_006248516.1 NZ_CP097334:c306043-305711 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT245182 WP_006248517.1 NZ_CP097334:c306300-306040 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References