Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2053069..2053719 Replicon chromosome
Accession NZ_CP097333
Organism Mannheimia haemolytica strain 184

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag M3709_RS10065 Protein ID WP_006249411.1
Coordinates 2053069..2053458 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag M3709_RS10070 Protein ID WP_006251766.1
Coordinates 2053459..2053719 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3709_RS10015 (M3709_10015) 2048424..2049233 - 810 WP_006250123.1 metal ABC transporter permease -
M3709_RS10020 (M3709_10020) 2049226..2050086 - 861 WP_006250124.1 metal ABC transporter permease -
M3709_RS10025 (M3709_10025) 2050160..2050945 - 786 WP_006251768.1 hypothetical protein -
M3709_RS10060 (M3709_10060) 2051811..2053049 - 1239 WP_006251767.1 peptidase T -
M3709_RS10065 (M3709_10065) 2053069..2053458 - 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
M3709_RS10070 (M3709_10070) 2053459..2053719 - 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M3709_RS10075 (M3709_10075) 2053835..2054047 - 213 WP_006251765.1 BrnA antitoxin family protein -
M3709_RS10080 (M3709_10080) 2054048..2054335 - 288 WP_006251764.1 BrnT family toxin -
M3709_RS10085 (M3709_10085) 2054498..2058031 - 3534 WP_040081125.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T245180 WP_006249411.1 NZ_CP097333:c2053458-2053069 [Mannheimia haemolytica]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T245180 NZ_CP097333:c2053458-2053069 [Mannheimia haemolytica]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT245180 WP_006251766.1 NZ_CP097333:c2053719-2053459 [Mannheimia haemolytica]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT245180 NZ_CP097333:c2053719-2053459 [Mannheimia haemolytica]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References