Detailed information of TA system TA024518

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5220290..5221124
• Replicon: chromosome
• Accession: NC_013716
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D2TSS5
• Locus tag: ROD_RS24425
• Protein ID: WP_012908801.1
• Coordinates: 5220290..5220667 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: D2TSS6
• Locus tag: ROD_RS24430
• Protein ID: WP_012908802.1
• Coordinates: 5220756..5221124 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS24395	5216593..5217015	+	423	Protein_4811	hypothetical protein	-
ROD_RS24400	5217038..5217226	+	189	WP_012908798.1	helix-turn-helix domain-containing protein	-
ROD_RS28680	5217465..5217792	+	328	Protein_4813	integrase domain-containing protein	-
ROD_RS27830	5217788..5218390	-	603	Protein_4814	integrase arm-type DNA-binding domain-containing protein	-
ROD_RS24410	5218664..5219506	-	843	Protein_4815	DUF4942 domain-containing protein	-
ROD_RS24415	5219591..5219788	-	198	WP_012908799.1	DUF957 domain-containing protein	-
ROD_RS24420	5219805..5220293	-	489	WP_012908800.1	hypothetical protein	-
ROD_RS24425	5220290..5220667	-	378	WP_012908801.1	TA system toxin CbtA family protein	Toxin
ROD_RS24430	5220756..5221124	-	369	WP_012908802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ROD_RS24435	5221174..5221818	-	645	WP_012908803.1	hypothetical protein	-
ROD_RS24440	5221837..5222058	-	222	WP_000692323.1	DUF987 domain-containing protein	-
ROD_RS24445	5222121..5222597	-	477	WP_001186727.1	RadC family protein	-
ROD_RS24450	5222613..5223098	-	486	WP_012908804.1	antirestriction protein	-
ROD_RS24455	5223189..5224007	-	819	WP_012908805.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14182.13 Da
• Isoelectric Point: 6.8436

>T24518 WP_012908801.1 NC_013716:c5220667-5220290 [Citrobacter rodentium ICC168]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNDITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T24518 NC_013716:c5220667-5220290 [Citrobacter rodentium ICC168]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAACAGCATATTG
AGGCGGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCTCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13829.62 Da
• Isoelectric Point: 6.9465

>AT24518 WP_012908802.1 NC_013716:c5221124-5220756 [Citrobacter rodentium ICC168]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT24518 NC_013716:c5221124-5220756 [Citrobacter rodentium ICC168]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: D2TSS5

Antitoxin

• AlphaFold DB: D2TSS6