Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 286662..287251 Replicon chromosome
Accession NZ_CP097333
Organism Mannheimia haemolytica strain 184

Toxin (Protein)


Gene name pemK Uniprot ID A0A248ZY19
Locus tag M3709_RS01445 Protein ID WP_006248516.1
Coordinates 286662..286994 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A248ZX26
Locus tag M3709_RS01450 Protein ID WP_006248517.1
Coordinates 286991..287251 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3709_RS01405 (M3709_01405) 281767..282801 - 1035 WP_006248509.1 endolytic transglycosylase MltG -
M3709_RS01425 (M3709_01425) 283381..283908 + 528 WP_006248512.1 inorganic diphosphatase -
M3709_RS01430 (M3709_01430) 284005..284658 - 654 WP_040080515.1 IS1595 family transposase -
M3709_RS01435 (M3709_01435) 284730..285440 + 711 WP_052437178.1 M48 family metallopeptidase -
M3709_RS01440 (M3709_01440) 285504..286535 - 1032 WP_006248514.1 tryptophan--tRNA ligase -
M3709_RS01445 (M3709_01445) 286662..286994 - 333 WP_006248516.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M3709_RS01450 (M3709_01450) 286991..287251 - 261 WP_006248517.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M3709_RS01455 (M3709_01455) 287321..287989 - 669 WP_006248518.1 phosphoglycolate phosphatase -
M3709_RS01460 (M3709_01460) 288076..288873 - 798 WP_021279892.1 prolipoprotein diacylglyceryl transferase -
M3709_RS01465 (M3709_01465) 288881..289669 - 789 WP_006248520.1 sulfite exporter TauE/SafE family protein -
M3709_RS01470 (M3709_01470) 289671..290315 - 645 WP_006248521.1 RNA pyrophosphohydrolase -
M3709_RS01475 (M3709_01475) 290927..292072 + 1146 WP_040080565.1 FMN-dependent L-lactate dehydrogenase LldD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 284005..284658 653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin

SpoVT-AbrB domain

(7-48)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12007.94 Da        Isoelectric Point: 6.4597

>T245176 WP_006248516.1 NZ_CP097333:c286994-286662 [Mannheimia haemolytica]
MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVPITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTV
DIAQRHGKFIEALPESLLDEIMARVSVIFE

Download         Length: 333 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9418.88 Da        Isoelectric Point: 4.7228

>AT245176 WP_006248517.1 NZ_CP097333:c287251-286991 [Mannheimia haemolytica]
MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVINPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPA
IGKEIL

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A248ZY19

Antitoxin

Source ID Structure
AlphaFold DB A0A248ZX26

References