Detailed information of TA system TA024517

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5198138..5198792
• Replicon: chromosome
• Accession: NC_013716
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: D2TS18
• Locus tag: ROD_RS24310
• Protein ID: WP_012908780.1
• Coordinates: 5198385..5198792 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: D2TS17
• Locus tag: ROD_RS24305
• Protein ID: WP_012908779.1
• Coordinates: 5198138..5198404 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS24280	5194059..5195493	-	1435	Protein_4790	6-phospho-beta-glucosidase	-
ROD_RS24285	5195683..5195994	+	312	WP_012908776.1	N(4)-acetylcytidine aminohydrolase	-
ROD_RS24290	5196157..5196819	+	663	WP_012908777.1	hemolysin III family protein	-
ROD_RS24295	5196901..5197881	-	981	WP_012908778.1	tRNA-modifying protein YgfZ	-
ROD_RS24305	5198138..5198404	+	267	WP_012908779.1	FAD assembly factor SdhE	Antitoxin
ROD_RS24310	5198385..5198792	+	408	WP_012908780.1	protein YgfX	Toxin
ROD_RS24315	5198836..5199357	-	522	WP_012908781.1	flavodoxin FldB	-
ROD_RS24320	5199458..5200354	+	897	WP_012908782.1	site-specific tyrosine recombinase XerD	-
ROD_RS24325	5200378..5201091	+	714	WP_012908783.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
ROD_RS24330	5201097..5202830	+	1734	WP_012908784.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-77)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15724.61 Da
• Isoelectric Point: 9.4464

>T24517 WP_012908780.1 NC_013716:5198385-5198792 [Citrobacter rodentium ICC168]
VVLWQSDLRVSWRAQWLSLLLHGLVAAAILLMPWPLSYTPLWLLLLSLVVFDCVRSQRRINACQGEVKLLMDGRLHWQGQ
DWSVISAPWMMKAGMMLRLRSDGGKRQHLWIAADSMDEAEWRDLCRLMLQPSTQR

Nucleotide

• Length: 408 bp

>T24517 NC_013716:5198385-5198792 [Citrobacter rodentium ICC168]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCATGGCGCGCGCAGTGGCTGTCGCTACTCCTGCATGGCCTGGTGGCCGC
GGCGATTTTATTAATGCCTTGGCCGTTGAGTTACACGCCGCTGTGGTTGTTATTACTTTCACTGGTCGTGTTTGACTGCG
TTCGCAGTCAGCGGCGAATTAATGCCTGTCAGGGCGAGGTGAAACTGCTGATGGATGGCCGGCTGCACTGGCAGGGGCAG
GACTGGTCGGTTATCAGCGCCCCGTGGATGATGAAAGCCGGGATGATGCTGCGCCTGCGTTCAGACGGCGGTAAACGCCA
GCACCTGTGGATCGCGGCGGACAGCATGGATGAGGCGGAGTGGCGCGATCTCTGCCGCCTGATGCTGCAACCGTCGACGC
AGCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10498.08 Da
• Isoelectric Point: 5.6311

>AT24517 WP_012908779.1 NC_013716:5198138-5198404 [Citrobacter rodentium ICC168]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDAEKRIFVRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQIRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT24517 NC_013716:5198138-5198404 [Citrobacter rodentium ICC168]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGCCGTCGCGGTATGCGTGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAATACGACACGTTAAGCGATGCGGAAAAACGCATTTTCGTCCGCCTGCTGGAGTGTGACGATCCAGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCAGATGCTGAACTGGAGCAGATGGTACGACTCATCCAGATACGGAAT
CGGGATCGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: D2TS18

Antitoxin

• AlphaFold DB: D2TS17