Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1241205..1242122 Replicon chromosome
Accession NZ_CP097328
Organism Bacillus velezensis strain ES2-4

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag M5L76_RS06495 Protein ID WP_007407256.1
Coordinates 1241376..1242122 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag M5L76_RS06490 Protein ID WP_003154807.1
Coordinates 1241205..1241375 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5L76_RS06450 (M5L76_06450) 1236438..1238060 + 1623 WP_032874601.1 pyocin knob domain-containing protein -
M5L76_RS06455 (M5L76_06455) 1238073..1238444 + 372 WP_032874603.1 XkdW family protein -
M5L76_RS06460 (M5L76_06460) 1238449..1238646 + 198 WP_032874605.1 XkdX family protein -
M5L76_RS06465 (M5L76_06465) 1238703..1239464 + 762 WP_032874607.1 hypothetical protein -
M5L76_RS06470 (M5L76_06470) 1239516..1239779 + 264 WP_003154815.1 hemolysin XhlA family protein -
M5L76_RS06475 (M5L76_06475) 1239793..1240056 + 264 WP_003154813.1 phage holin -
M5L76_RS06480 (M5L76_06480) 1240070..1240948 + 879 WP_032874609.1 N-acetylmuramoyl-L-alanine amidase -
M5L76_RS06485 (M5L76_06485) 1240983..1241108 - 126 WP_003154809.1 hypothetical protein -
M5L76_RS06490 (M5L76_06490) 1241205..1241375 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
M5L76_RS06495 (M5L76_06495) 1241376..1242122 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
M5L76_RS06500 (M5L76_06500) 1242227..1243225 - 999 WP_032874611.1 inorganic phosphate transporter -
M5L76_RS06505 (M5L76_06505) 1243238..1243855 - 618 WP_032874614.1 DUF47 domain-containing protein -
M5L76_RS06510 (M5L76_06510) 1244141..1245457 - 1317 WP_032874616.1 amino acid permease -
M5L76_RS06515 (M5L76_06515) 1245779..1246729 + 951 WP_032874618.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1197451..1299974 102523


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T245161 WP_007407256.1 NZ_CP097328:c1242122-1241376 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT245161 WP_003154807.1 NZ_CP097328:c1241375-1241205 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References