245160

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 489965..490602 Replicon chromosome
Accession NZ_CP097328
Organism Bacillus velezensis strain ES2-4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M5L76_RS02510 Protein ID WP_003156187.1
Coordinates 490252..490602 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M5L76_RS02505 Protein ID WP_003156188.1
Coordinates 489965..490246 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5L76_RS02485 (M5L76_02485) 486330..486929 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
M5L76_RS02490 (M5L76_02490) 487022..487387 + 366 WP_007609580.1 holo-ACP synthase -
M5L76_RS02495 (M5L76_02495) 487552..488559 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
M5L76_RS02500 (M5L76_02500) 488676..489845 + 1170 WP_032873001.1 alanine racemase -
M5L76_RS02505 (M5L76_02505) 489965..490246 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M5L76_RS02510 (M5L76_02510) 490252..490602 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M5L76_RS02515 (M5L76_02515) 490720..491541 + 822 WP_003156182.1 STAS domain-containing protein -
M5L76_RS02520 (M5L76_02520) 491546..491911 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M5L76_RS02525 (M5L76_02525) 491914..492315 + 402 WP_003156178.1 anti-sigma regulatory factor -
M5L76_RS02530 (M5L76_02530) 492327..493334 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
M5L76_RS02535 (M5L76_02535) 493398..493727 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M5L76_RS02540 (M5L76_02540) 493724..494206 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M5L76_RS02545 (M5L76_02545) 494172..494960 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
M5L76_RS02550 (M5L76_02550) 494960..495562 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245160 WP_003156187.1 NZ_CP097328:490252-490602 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245160 WP_003156188.1 NZ_CP097328:489965-490246 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References