Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508756..509393 Replicon chromosome
Accession NZ_CP097326
Organism Bacillus velezensis strain GUCC45

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M5J22_RS02455 Protein ID WP_003156187.1
Coordinates 509043..509393 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M5J22_RS02450 Protein ID WP_003156188.1
Coordinates 508756..509037 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5J22_RS02430 (M5J22_02430) 505121..505720 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
M5J22_RS02435 (M5J22_02435) 505813..506178 + 366 WP_003156192.1 holo-ACP synthase -
M5J22_RS02440 (M5J22_02440) 506343..507350 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
M5J22_RS02445 (M5J22_02445) 507467..508636 + 1170 WP_003156189.1 alanine racemase -
M5J22_RS02450 (M5J22_02450) 508756..509037 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M5J22_RS02455 (M5J22_02455) 509043..509393 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M5J22_RS02460 (M5J22_02460) 509511..510332 + 822 WP_003156182.1 STAS domain-containing protein -
M5J22_RS02465 (M5J22_02465) 510337..510702 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M5J22_RS02470 (M5J22_02470) 510705..511106 + 402 WP_201488789.1 anti-sigma regulatory factor -
M5J22_RS02475 (M5J22_02475) 511118..512125 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
M5J22_RS02480 (M5J22_02480) 512189..512518 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M5J22_RS02485 (M5J22_02485) 512515..512997 + 483 WP_003156173.1 anti-sigma B factor RsbW -
M5J22_RS02490 (M5J22_02490) 512963..513751 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M5J22_RS02495 (M5J22_02495) 513751..514353 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245153 WP_003156187.1 NZ_CP097326:509043-509393 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245153 WP_003156188.1 NZ_CP097326:508756-509037 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References